What is laminar air flow system?
A laminar flow cabinet or laminar
flow closet or tissue culture hood is a carefully enclosed bench designed to
prevent contamination of semiconductor wafers, biological samples, or any
particle sensitive materials. Air is drawn through a HEPA filter and blown in a
very smooth, laminar flow towards the user.
What is a biological safety cabinet?
A biosafety cabinet (BSC) — also
called a biological safety cabinet or microbiological safety cabinet — is an
enclosed, ventilated laboratory workspace for safely working with materials
contaminated with (or potentially contaminated with) pathogens requiring a
defined biosafety level.
What is the biosafety?
Biosafety is the prevention of
large-scale loss of biological integrity, focusing both on ecology and human
health. These prevention mechanisms include conduction of regular reviews of
the biosafety in laboratory settings, as well as strict guidelines to follow.
Biosafety is used to protect us from harmful incidents.
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SOP
for Laminar Air Flow Unit
1.0
OBJECTIVE:
To lay down a Procedure is to provide
guidelines for operation of Laminar Air Flow.
2.0
SCOPE:
This procedure is applicable for LAF
which is installed in Microbiology laboratory of QC Department
3.0
RESPONSIBILITY:
Microbiologist
4.0
ACCOUNTABILITY:
HEAD QA & QC
5.0
PROCEDURE:
5.1
Operation & Cleaning of LAF
5.1.1 Switch “OFF” the UV light.
5.1.2 Clean the equipment with clean lint free
cloth duster and spray 70% IPA solution to LAF unit and entire Area of LAF
room.
5.1.3 Switch “ON” the “AIR” and Visible Light
switch situated on the control panel on right hand side of the instrument.
5.1.4 Now check the LAF Manometer pressure, it
should be within 10 – 20 mm of Water Gauge.
5.1.5 Check the log record for burning hour of UV
light, it should not exceed more than 2000 hours. UV tube should be replaced
after specified burning period.
5.1.6 Start the gas burner with the help of gas
lighter and carry out the routine works.
5.1.7 After completion of work, switch OFF the
Airflow and gas burner.
5.1.8 Clean any remaining water or waste liquid
material spilled on the laminar airflow platform properly with a dry cloth.
5.1.9 Clean properly the working chamber from
ceiling, followed by side glass and then the platform of the chamber with
sterile 70% IPA.
5.1.10 Spray sterile 70% IPA and switch OFF the
Visible light and then Switch ON the UV light till to start the next operation.
5.1.11 If the LAF is used for Aseptic filtration,
clean the receiver pipe tank with hot WFI.
5.1.12 Open the outlet of Solution Collection Tank
and collect the solution in plastic crate. Close the valve and transfer the
crate for solution disposal.
5.2
Cleaning of Pre filters
5.2.1 Ensure that the LAF is switch off. Affix the
tag on Instrument “UNDER MAINTENANCE”.
5.2.2 Pre filter is situated on the backside of the
LAF, unscrew and then remove the pre filter from LAF.
5.2.3 Transfer the pre filter to washing area and
blow the compressed air from reverse side to blow out all the dust particles.
5.2.4 Wash the pre filter with DM water and then
with liquid detergent solution. Finally wash again with DM water to remove the
detergent solution.
5.2.5 Remove the trapped water from the pre filter
by jerking and finally rinse with 70% IPA solution.
5.2.6 Allow to dry the pre filter at room
temperature.
5.2.7 Assemble the pre-filter at their original
place and tighten the screw properly.
5.3
Precautions
5.3.1 Take care to prevent any damage to the
integrity of filter during cleaning. In case of observation of any damage to
filters, immediately inform to your superior for further action.
5.3.2 Instrument should be cleaned when the
electrical connections of the equipment is in OFF position.
5.3.3 Clean the laminar airflow chamber after every
operation.
5.3.4 Do not work when UV light is ON as it may cause
eye damage.
5.3.5 Maintain the level of the platform by
adjusting the equipment from the base with the help of glass beads in such a
manner that the platform level should remain horizontally flat. There should be
no ups and downs in any portion of the chamber.
5.4
Frequencies
5.4.1 Daily for operation
5.4.2 Fortnightly for Pre filter cleaning
5.4.3 Calibration: Yearly by External agency
6.0
ABBREVIATIONS:
SOP : Standard Operating Procedure
QA : Quality Assurance
QC : Quality Control
NA : Not Applicable
LAF : Laminar Air Flow
IPA : Isopropyl Alcohol
DM : Dematerialized
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ReplyDeleteAcmas Technologies Inc is leading laminar airflow cabinet or vertical autoclave manufacturer in India.
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ReplyDeleteIf you want to know more about the Laminar Air Flow, please visit our website:
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