Biochemical Test and Identification of E. coli
| Characteristics | E. coli |
|---|---|
| Gram Staining | Negative |
| Shape (Cocci/Diplococci/Rods) | Rods |
| Motility (Motile / Non-Motile) | Motile |
| Capsule (Capsulated/Non-Capsulated) | Variable |
| Spore (Sporing/Non-Sporing) | Non-Sporing |
| Flagella (Flagellated/Non-Flagellated) | Flagellated |
| Catalase | Positive (+ve) |
| Oxidase | Negative (-ve) |
| MR | Positive (+ve) |
| VP | Negative (-ve) |
| OF (Oxidative/Fermentative) | Fermentative |
| Indole | Positive (+ve) |
| Citrate | Negative (-ve) |
| Urease | Negative (-ve) |
| Nitrate Reduction | Positive (+ve) |
| H2S | Negative (-ve) |
| Gas | Positive (+ve) |
| PYR | – |
| CAMP | – |
| Gelatin | Negative (-ve) |
| Niacin | – |
| Coagulase | – |
| Hemolysis (Alfa/Beta/Gamma) | Some Strains shows Hemolysis |
| Nagler | – |
| String Test | – |
| Pigment | – |
| Bile Solubility | – |
| Fermentation of | |
| Arabinose | Positive (+ve) |
| DNase | Negative (-ve) |
| Fructose | – |
| Glucose | Positive (+ve) |
| Hippurate | – |
| Inositol | Negative (+ve) |
| Inulin | – |
| Lactose | Positive (+ve) |
| Maltose | – |
| Mannitol | Positive (+ve) |
| Mannose | – |
| MyoInositol | – |
| Raffinose | – |
| Ribose | – |
| Sorbitol | Positive (+ve) |
| Sucrose | Variable |
| Enzymatic Reactions | |
| Acetate Utilization | Positive (+ve) |
| Acid Phosphatase | – |
| Alkaline Phosphatase | – |
| Amidase | – |
| Arginine dehydrolase | Variable |
| Arylsulphatase | – |
| Beta Lactamase | – |
| Hyalurodinase | – |
| Lecithinase | – |
| Lipase | – |
| Lysine | Positive (+ve) |
| Neuraminidase | – |
| ONPG Test | Positive (+ve) |
| Ornithine decarboxylase | Variable |
| Phenylalanine deaminase | Negative (-ve) |
| Perooxidase | – |
| Tellurite | – |
| Tributyrin | – |
| Differential Antibiotic | |
| Novobiocin | – |
| Bacitracin | – |
| Optochin | – |
| Streptomysin | – |
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