Friday, 18 July 2025

SOP for Cleaning and Operation of BOD Incubator (30°C - 35°C)

 SOP for Cleaning and Operation of BOD Incubator (30°C - 35°C)

Discussion with Shri LVV Rao Sir under Quality control improvement 

Please find the SOP for Cleaning and Operation of BOD Incubator (30°C - 35°C) and brief about the A KB-6 CFT BOD incubator typically refers to a 6 cubic feet Biological Oxygen Demand (BOD) incubator, commonly used in laboratories for testing water samples. Key specifications include a temperature range of 5°C to 75°C, a double-walled construction, and features like an air circulation fan and temperature control system. 

Power Supply issue may be .

Detailed Specifications:

Capacity: 6 cubic feet (approximately 170 liters). 

Temperature Range: Typically 5°C to 75°C, with some models offering a range down to -10°C for specific applications. 

Temperature Control: Microprocessor-based digital temperature controller for precise temperature setting and maintenance. 

Construction: Double-walled with insulation (often Mineral Wool or PUF) to maintain temperature stability and minimize heat loss. 

Inner Chamber: Usually made of stainless steel for corrosion resistance and easy cleaning. 

Outer Body: Can be made of mild steel with powder coating or stainless steel. 

Shelves: Perforated stainless steel shelves for sample placement, typically removable and adjustable. 

Door: Inner glass door for viewing samples without opening the main door, and an insulated outer door with a locking mechanism. 

Air Circulation: An air circulation fan to ensure uniform temperature distribution within the chamber. 

Cooling System: Includes a refrigeration system (often CFC-free) to maintain lower temperatures. 

Safety Features: Over-temperature safety thermostat, voltage stabilizer, and door lock for sample security. 

Power Supply: Typically 220-230V AC, single-phase. 

Display: Digital display for monitoring temperature and other parameters. 

Optional Features: UV lamp for sterilization, data logging system, and validation port. 

Note: Specific features and dimensions may vary slightly depending on the manufacturer and model.

SOP for Cleaning and Operation of BOD Incubator (30°C - 35°C)

Standard operating procedure to clean the BOD Incubator (30°C - 35°C).

1.0 OBJECTIVE

To ensure that the incubator performs satisfactorily and maintains accurate temperature for the growth of Microorganisms.

2.0 SCOPE

This SOP is applicable for the Procedure for operation of BOD incubator (30°C- 35°C)

3.0 RESPONSIBILITY

Microbiologist - Quality Control

4.0 ACCOUNTABILITY

Manager- Quality Control

5.0 PROCEDURE

5.1 General cleaning procedure

5.1.1 Ensure that the power supply to the incubator is switched OFF.

5.1.2 De-dust the incubator daily externally with a clean dry cloth.

5.1.3 Once in a week remove adhered dust by wet mopping using soap solution. Afterwards wipe the surface with a clean dry cloth to remove themoisture.

5.1.4 Mop the interior surfaces with a clean dry cloth daily.

5.2 Operating Procedure

5.2.1 Ensure that the incubator is properly connected to the power supply.


5.2.2 Switch ‘ON’ the main switch and then the cabinet switch.

5.2.3 Set the required temperature to 32.5 °C by pressing the 'set knob and soft keys.

5.2.4 Monitor the temperature daily as per following procedure.

5.2.5 Temperature shall be recorded which is displayed on LCD of controller of incubator.

5.2.6 Observe the temperature shown on digital display. The temperature should not differ by ±2 °C.

5.2.7 Temperature of incubator having online printer shall be recorded in printout chart.

5.2.8 Take the print out on monthly basis.

5.2.9 If the online recording system is not working properly inform Manager-QC and put 'Out of Order' label, in this condition only fill the manualrecord as per format.

5.2.10 Once the printer is rectified, a comment shall be written on the printout for the gap period.

5.2.11 Report any discrepancy observed during operation or temperature monitoring to Manager-QC.

5.2.12 Inform to Engineering Department for rectification and put the status label of 'Under Maintenance'.

5.2.13 Maintain the incubator usage log.

6.0 ABBREVIATIONS


6.1 SOP - Standard Operating Procedure


6.2 No. - Number


6.3 QA - Quality Assurance


6.4 CCF - Change control format


6.5 QC - Quality Control


6.6 NA - Not Applicable


SOP for Cleaning and Operation of BOD Incubator (30°C - 35°C) | 




With best regards,


Dr. Amar Nath Giri.

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