parameters and bioaccumulation of heavy metal in Pisum sativum
Amar Nath Giri*
Environment Management System, NFCL, Nagarjuna Road, Kakinada, India
*e-mail: amarnathgiri@nagarjunagroup.com, goswami818@yahoo.com
Abstract: The crude treated effluent was collected from pulp and paper industry from its outlet. Different concentration i.e. control (0), 10, 20, 50 and 100% were
prepared from the effluent using distilled water. Seeds of pea (Pisum sativum) were soaked in different concentrations of effluents and allowed to germinate in
sand pot. The germination percentage on 10th day was gradually decreased in various dilutions of treated effluents. The fresh wt., dry wt., chlorophyll contents
and protein content were increased with increase in dilution of treated pulp and paper mill effluent. However, the 100% of treated effluent were resulting in
retardation of growth. The effect on enzyme due to application of different dilutions of treated effluent showed that value of peroxidase and catalase were increased
with increase in concentration. The value of acid phosphtase was increased with increase in concentration till 50% of treated effluent while ribonuclease value
was significantly decreases with increase in concentration of treated effluent in 25% but increased in 50% and 100% of treated effluent concentration. Fe, Ni
and Cd concentration were increased significantly with increase in concentration of effluent.
Key words: Pulp and paper mill, Effluent, Chlorophyll, Acid phosphatase, Peroxidase, Catalase, Ribonuclease, Protein
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