The macronutrients composition

Equipment

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Our laboratory is equipped with:

-         3 plant growth rooms 

-         1 greenhouse space (68 m²)

-         a cold room

-         a room for bacterial culture at 37°C

-         a radioactive room

-         2 thermocyclers

-         a spectrophotometer NanoDrop 2000

-         table centrifuges and shaking water baths

-         1D electrophoresis equipment 

-        2D electrophoresis equipment (FNRS C.C. 1.5.038.03)

-         cDNA-AFLP required equipment

-         laminar flows (included one Biohasard model)

-         ultra low temperature freezer (-85°C, 240L)

-         2 phytotrons VOTSCH (VB104 + SGC.XXX.C)

-         automatic autoclave (industrial type)

-         2 incubator-shakers for cell culture (plant and yeast)

-         a research microscope (Nikon-Eclipse E800) and a stereomicroscope (Nikon-SMZ 1000) equipped for fluorescence studies, with a digital acquisition system (Nikon-DXM 1200). 

  

Culture facilities

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Green house (surface 68 m2)	Hydroponic culture of Arabidopsis halleri

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Phytotron	Hydroponic culture of Thlaspi caerulescens

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In vivo culture room	Hydroponic culture of Beta vulgaris
Hydroponic culture of Arabidopsis thaliana

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In vitro growth rooms	In vitro growth of Arabidopsis thaliana

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Growth conditions - Hydroponics culture

Arabidopsis thaliana

Seeds of Arabidopsis thaliana are germinated in soil. Three weeks after sowing at 8H (100µmol/m2s) light / 16 H dark,  plantlets are transferred into containers for hydroponics culture. The macronutrients composition is in mM:  1,00 Ca(NO3) 2,  1,00 MgSO4,  0,88 K2SO4,  0,25 KH2PO4, 0,01 NaCl and the micronutrients composition in µM is  20,00 FeEDTA,  ,  10,00 H3BO3,   1,00 ZnSO4,  1,00 MnSO4,  0,10 CuSO4,  0,01 (NH4)6Mo7O24. The pH of the solution is adjusted to 5,8 ± 0,1.  To know more about different hydroponics systems: http://gcramer-mac.ag.unr.edu/hydroponic.html Gibeaut J.M. et al.,  Maximal biomass of Arabidopsis thaliana using a simple, low maintenance hydroponic method and favorable environmental conditions. Plant Physiol (1997) 115:317-319.

Arabidopsis halleri

Beta vulgaris

cv Adonis

Seeds of Beta vulgaris are germinated in soil. Three weeks after sowing,  plantlets are transferred into containers for hydroponics culture. The macronutrients composition is in mM: 2,00 Ca(NO3) 2, 1,00 MgSO4, 0,88 K2SO4, 0,25 KH2PO4 and the micronutrients composition in µM is 20 FeEDTA, 10 NaCl, 10 H3BO3,  1,00 ZnSO4, 1,00 MnSO4, 0,10 CuSO4, 0,01 (NH4)6Mo7O24. The pH of the solution is adjusted to 5,8 ± 0,1. In the Mg deficiency experiments, plants are fed with a Mg-free nutrient solution, which is the same as above formulated, except that  1,00 mM MgSO4 was replaced by 1,00 mM Na2SO4. During all the experiment, nutrient solution are replaced every 4 days. The growth conditions in the culture room are 16 H light (300 µE m-2 s-1) / 8 H dark photoperiodism, temperature 23°C.

Thlaspi caerulescens - Thlaspi arvense

Seeds of Thlaspi  are germinated on sterilized and moistened sand. After germination, the sand is moistened with 0,2-strength nutrient solution (see below). Five to seven days after germination, seedlings are transferred to 1,2 l vessels (10 plants per vessel) containing a  nutrient solution (Basic "modified" 0,1 strength Hoagland's solution) which composition is in mM: 0,40 Ca(NO3) 2, 0,20 MgSO4, 0,50 KNO3, 0,10 KH2PO4 and the micronutrients composition in µM is 20 FeEDDHA,  10,00 H3BO3,  10,00 ZnSO4, 2,00 MnSO4, 0,20 CuSO4, 0,01 Na2MoO4. Plants are grown in a glasshouse with supplementary illumination provided by sodium-vapour lamps to give a photoperiod of 16 H per day. The nutrient solution is aerated continuously and renewed every seven days.