Equipment
Our laboratory is equipped with:
-
3 plant growth rooms
-
1 greenhouse space (68 m2)
-
a cold room
-
a room for bacterial culture at 37°C
-
a radioactive room
- 2 thermocyclers
-
a spectrophotometer NanoDrop 2000
-
table centrifuges and shaking water baths
-
1D electrophoresis equipment
- 2D electrophoresis equipment
(FNRS C.C. 1.5.038.03)
-
cDNA-AFLP required equipment
-
laminar flows (included one Biohasard model)
-
ultra low temperature freezer (-85°C, 240L)
- 2 phytotrons VOTSCH (VB104 + SGC.XXX.C)
-
automatic autoclave (industrial type)
-
2 incubator-shakers for cell culture (plant and yeast)
-
a research
microscope (Nikon-Eclipse E800) and a stereomicroscope (Nikon-SMZ 1000) equipped
for fluorescence studies, with a digital acquisition system (Nikon-DXM
1200).
|
Green house
(surface 68 m2)
|
Hydroponic
culture of Arabidopsis halleri
|
 |
 |
 |
 |
 |
|
|
Phytotron
|
Hydroponic culture of Thlaspi
caerulescens
|
|
 |
 |
 |
|
In
vivo culture room
|
Hydroponic culture of Beta
vulgaris
|
 |
 |
|
Hydroponic culture of Arabidopsis
thaliana
|
|
 |
 |
|
In
vitro growth
rooms
|
In vitro growth of Arabidopsis
thaliana
|
 |
 |
Growth conditions - Hydroponics culture
|
Arabidopsis thaliana
|
|
 |
Seeds of Arabidopsis thaliana are germinated in soil. Three weeks
after sowing at 8H (100µmol/m2s) light / 16 H dark, plantlets are
transferred into containers for hydroponics culture. The macronutrients
composition is in mM: 1,00 Ca(NO3) 2, 1,00
MgSO4, 0,88 K2SO4, 0,25
KH2PO4, 0,01 NaCl and the micronutrients composition in µM
is 20,00 FeEDTA, , 10,00 H3BO3, 1,00 ZnSO4, 1,00
MnSO4, 0,10 CuSO4, 0,01
(NH4)6Mo7O24. The pH of the solution
is adjusted to 5,8 ± 0,1.
To know more about different hydroponics systems:
http://gcramer-mac.ag.unr.edu/hydroponic.html
Gibeaut J.M. et al., Maximal biomass of Arabidopsis thaliana
using a simple, low maintenance hydroponic method and favorable environmental
conditions. Plant Physiol (1997)
115:317-319.
|
|
Arabidopsis halleri
|
|
|
|
Beta vulgaris
|
cv
Adonis
|
 |
Seeds of
Beta vulgaris are germinated in soil. Three weeks after sowing,
plantlets are transferred into containers for hydroponics culture. The
macronutrients composition is in mM: 2,00 Ca(NO3) 2, 1,00
MgSO4, 0,88 K2SO4, 0,25
KH2PO4 and the micronutrients composition in µM is 20
FeEDTA, 10 NaCl, 10 H3BO3, 1,00
ZnSO4, 1,00 MnSO4, 0,10 CuSO4, 0,01
(NH4)6Mo7O24. The pH of the solution
is adjusted to 5,8 ± 0,1. In the Mg deficiency experiments, plants are fed with
a Mg-free nutrient solution, which is the same as above formulated, except
that 1,00 mM
MgSO4 was replaced by 1,00 mM Na2SO4.
During
all the experiment, nutrient solution are replaced every 4 days. The growth
conditions in the culture room are 16 H light (300
µE
m-2 s-1) / 8 H dark photoperiodism, temperature
23°C.
|
|
Thlaspi caerulescens - Thlaspi
arvense
|
|
 |
Seeds of Thlaspi are germinated on
sterilized and moistened sand. After germination, the sand is moistened with
0,2-strength nutrient solution (see below). Five to seven days after
germination, seedlings are transferred to 1,2 l vessels (10 plants per vessel)
containing a nutrient solution (Basic
"modified" 0,1 strength Hoagland's solution) which composition is in mM: 0,40
Ca(NO3) 2, 0,20 MgSO4, 0,50 KNO3,
0,10 KH2PO4 and the micronutrients composition in µM is 20
FeEDDHA, 10,00 H3BO3, 10,00
ZnSO4, 2,00 MnSO4, 0,20 CuSO4, 0,01
Na2MoO4. Plants are grown in a glasshouse with
supplementary illumination provided by sodium-vapour lamps to give a photoperiod
of 16 H per day.
The nutrient solution is aerated continuously and renewed every seven
days.
|
No comments:
Post a Comment